bs-3494R-BF647
[Conjugated Primary Antibody]
mTOR (Ser2448) Polyclonal Antibody, AbBy Fluor® 647 Conjugated
DATASHEET
Host: Rabbit
Target Protein: mTOR Ser2448
Specificity: This phosphorylation site is homologous across the listed species.
Modification Site: Ser2448
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2475
Swiss Prot: P42345
Source: KLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2448
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1.
Conjugation: AbBy Fluor® 647
Excitation/ Emission: 650nm/665nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 289
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Fish
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yang, Wei‐Rong, et al. "mTOR is involved in 17β‐estradiol‐induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." Molecular Reproduction and Development (2015).Read more>>
- Mei H et al. Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells.(2018)Int. J. Mol. Med.Nov;42(5):2676-2688. Read more>>
- Qiu J et al. Distinct subgroup of the Ras family member 3 (DIRAS3) expression impairs metastasis and induces autophagy of gastric cancer cells in mice.J Cancer Res Clin Oncol. 2018 Oct;144(10):1869-1886.Read more>>
- Mei et al. Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells. (2018) Int.J.Mol.Med. 42:2676-2688Read more>>
- Zhao H et al. The cardiotoxicity of the common carp (Cyprinus carpio) exposed to environmentally relevant concentrations of arsenic and subsequently relieved by zinc supplementation. Environmental Pollution.2019 Oct; 253:741-748.Read more>>
- Liao XD et al. Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells. Asian-Australas J Anim Sci. 2019 Aug 23. Read more>>
- Zhong et al. Camellia (Camellia oleifera Abel.) seed oil promotes milk fat and protein synthesis‐related gene expression in bovine mammary epithelial cells. Food Science & Nutrition. 2019.Read more>>
- Qi H et al. MSTN attenuates cardiac hypertrophy through inhibition of excessive cardiac autophagy by blocking AMPK/mTOR and miR-128/PPARγ/NF-κB signaling pathways. Mol Ther Nucleic Acids. 2019 Dec 14;19:507-522. Read more>>
- Xue W et al. Caveolin-1 alleviates lipid accumulation in NAFLD associated with promoting autophagy by inhibiting the Akt/mTOR pathway. Eur J Pharmacol. 2020 Mar 15;871:172910. Read more>>
- Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2). Read more>>
- Yu Wang. et al. Environmentally relevant concentration of sulfamethoxazole-induced oxidative stress-cascaded damages in the intestine of grass carp and the therapeutic application of exogenous lycopene. Environ Pollut. 2021 Apr;274:116597Read more>>
- Wang Ji-sheng. et al. A Bioinformatic Investigation of the Mechanism Underlying Migraine-Induced Erectile Dysfunction. Biomed Res Int. 2021;2021:6674643Read more>>
- eina Hui. et al. Silencing UHRF1 Enhances Radiosensitivity of Esophageal Squamous Cell Carcinoma by Inhibiting the PI3K/Akt/mTOR Signaling Pathway. Cancer Manag Res. 2021; 13: 4841C4852Read more>>
- Qi Hao. et al. Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway. Brit J Nutr. 2021 Dec;:1-27Read more>>
- Zong, Jinxin. et al. Lithium Chloride Promotes Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells via HIF-1_ and _-Catenin Signaling Pathways. Biol Trace Elem Res. 2022 Jan;:1-16Read more>>
- ai Baoxin. et al. Prognostic and functional roles of EIF4G1 in lung squamous cell carcinoma. HUM CELL. 2023 Mar;:1-9Read more>>
- Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26Read more>>
- Tingkui Zhao. et al. A Triple-Targeted Rutin-Based Self-Assembled Delivery Vector for Treating Ischemic Stroke by Vascular Normalization and Anti-Inflammation via ACE2/Ang1-7 Signaling. ACS CENTRAL SCI. 2023;XXXX(XXX):XXX-XXXRead more>>
- Jing Zhang. et al. Effects of dietary folic acid supplementation on lactation performance and mammary epithelial cell development of dairy cows and its regulatory mechanism. ANIM BIOTECHNOL. 2023 Jul 06Read more>>
- Hao Qi. et al. Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1B-UBR5-ARID1A Signaling. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
- Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-B-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11Read more>>
VALIDATION IMAGES