bs-3485R-BF350 [Conjugated Primary Antibody]
NFKB p65(Ser468) Polyclonal Antibody, AbBy Fluor® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKB p65 Ser468

Specificity: This phosphorylation site is homologous in Rat and equivalent to Ser467 in Mouse.

Modification Site: Ser468

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5970

Swiss Prot: Q04206

Source: KLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser468

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).

Conjugation: AbBy Fluor® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cheng Z et al. Cotinine inhibits TLR4/NF-B signaling pathway and improves deep vein thrombosis in rats. Biosci Rep. 2020 Jun 26;40(6):BSR20201293.Read more>>
  • Huajun Huang. et al. Effect of bone marrow mesenchymal stem cell transplantation combined with lugua polypeptide injection on osteoarthritis in rabbit knee joint. 2021 Aug 06Read more>>
  • Li, Lu. et al. Luteolin alleviates inflammation and autophagy of hippocampus induced by cerebral ischemia/reperfusion by activating PPAR gamma in rats. BMC Complementary Medicine and Therapies. 2022 Dec;22(1):1-13Read more>>
  • Yanjie Wang. et al. Inhibitory Effect of Bisdemethoxycurcumin on DNCB-Induced Atopic Dermatitis in Mice. MOLECULES. 2023 Jan;28(1):293Read more>>
  • Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
  • Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 ApRead more>>
  • Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-B signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192Read more>>
  • Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1C7) axis via NF-B/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11Read more>>
  • Fengjuan Chen. et al. JNK molecule is a toxic target for IPEC-J2 cell barrier damage induced by T-2 toxin. ECOTOX ENVIRON SAFE. 2023 Sep;263:115247Read more>>
  • Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
  • Wenyue Qiu. et al. Tert-butylhydroquinone attenuates LPS-induced pyroptosis of IPEC-J2 cells via downregulating HMGB1/TLR4/NF-B axis. J ANIM PHYSIOL AN N. 2023 SepRead more>>
  • Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
  • Hong-Fei Wang. et al. Jujuboside B alleviates acetaminophen-induced hepatotoxicity in mice by regulating Nrf2-STING signaling pathway. ECOTOX ENVIRON SAFE. 2024 Jan;269:11581Read more>>
  • Lei Liu. et al. Myricetin Oligomer Triggers Multi-Receptor Mediated Penetration and Autophagic Restoration of Blood-Brain Barrier for Ischemic Stroke Treatment. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Dating Pei. et al. Modulation of macrophage polarization by secondary cross-linked hyaluronan-dopamine hydrogels. INT J BIOL MACROMOL. 2024 Jun;270:132417Read more>>
  • Xue Gao. et al. Hydrogen therapy promotes macrophage polarisation to the M2 subtype in radiation lung injury by inhibiting the NF-B signalling pathway. HELIYON. 2024 1Read more>>
  • Sun Xuri. et al. Exogenous NT-3 Promotes Phenotype Switch of Resident Macrophages and Improves Sciatic Nerve Injury through AMPK/NF-B Signaling Pathway. NEUROCHEM RES. 2024 Jun;:1-15Read more>>
  • Yanan Wang. et al. Biomimetic Trypsin-Responsive Structure-Bridged Mesoporous Organosilica Nanomedicine for Precise Treatment of Acute Pancreatitis. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Kaixin Wang. et al. Long-Term Alcohol Exposure Aggravates Ischemic Stroke-Induced Damage by Promoting Pericyte NLRP3 Inflammasome Activation via Pre-Activating the TLR4/NF-B Pathway in Rats. J INFLAMM RES. 2024 23Read more>>
  • Zhengqun Liu. et al. The protective effect of dulcitol on lipopolysaccharide-induced intestinal injury in piglets: mechanistic insights. J NUTR BIOCHEM. 2024 Aug;:109719Read more>>
VALIDATION IMAGES