bs-3484R-PE [Conjugated Primary Antibody]
Smad2/3 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad2/3

Immunogen Range: 31-80/425


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4087

Swiss Prot: Q15796

Source: KLH conjugated synthetic peptide derived from human Smad2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Pig
Others

Predicted Cross Reactive Species: Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Lili, et al. "Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats." Frontiers in pharmacology 6 (2015).Read more>>
  • Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017).Read more>>
  • Bai, Long, et al. "BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling." Theriogenology (2017).Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
  • Wang et al. The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-β/Smad signaling and activation of the Nrf2/HO-1 pathway. (2016) Mol.Med.Re. 14:2389-96Read more>>
  • Hu et al. Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition. (2016) PLoS.On. 11:e0153409Read more>>
  • Zhou et al. Effects of Zearalenone Exposure on the TGF-β1/Smad3 Signaling Pathway and the Expression of Proliferation or Apoptosis Related Genes of Post-Weaning Gilts. (2018) Toxins.(Basel). 10 Read more>>
  • Zhou et al. Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway. (2018) Int.J.Mol.Med. 41:473-484Read more>>
  • Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
  • Wang et al. Effects of Tongxinluo on myocardial fibrosis in diabetic rats. (2016) J.Chin.Med.Assoc. 79:130-6Read more>>
  • Wang et al. Epigallocatechin-3-gallate attenuates unilateral ureteral obstruction-induced renal interstitial fibrosis in mice. (2015) J.Histochem.Cytochem. 63:270-9Read more>>
  • Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145. Read more>>
  • Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.Read more>>
  • Han B et al. Deltamethrin induces liver fibrosis in quails via activation of the TGF-β1/Smad signaling pathway. Environ Pollut. 2019 Dec 23;259:113870.Read more>>
  • Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-1/Smad3 pathwayBiomed Pharmacother.2020 Dec 24;134:111135.Read more>>
  • Xijuan Liu et al. Chondrocyte suppression is mediated by miR\129\5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip. J Orthop Res. 2020 Dec;38(12):2559-2572.Read more>>
  • Yang Fan. et al. Catalpol Protects Against Pulmonary Fibrosis Through Inhibiting TGF-1/Smad3 and Wnt/-Catenin Signaling Pathways. Front Pharmacol. 2021 Jan;11:2472Read more>>
  • Fei Yin. et al. Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on TGF-1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis Rats. Stem Cells Dev. 2020 Oct;29(21):1395-1406Read more>>
  • Yi Chen. et al. The essential oil from the raw and vinegar processed Rhizoma Curcumae ameliorate CCl4-incuded liver fibrosis: integrating network pharmacology and molecular mechanism evaluation. 2021 Mar 17Read more>>
  • Weimin Lin. et al. SESN3 Inhibited SMAD3 to Relieve Its Suppression for MiR-124, Thus Regulating Pre-Adipocyte Adipogenesis. Genes-Basel. 2021 Dec;12(12):1852Read more>>
  • Zhang Peng. et al. Study on the Mechanism of Bu-Shen-He-Mai Granules in Improving Renal Damage of Ageing Spontaneously Hypertensive Rats by Regulating Th17 Cell/Tregs Balance. EVID-BASED COMPL ALT. 2022;2022:8315503Read more>>
  • Cuifang Chang. et al. The orphan GPR50 receptor interacting with T_RI induces G1/S-phase cell cycle arrest via Smad3-p27/p21 in BRL-3A cells. BIOCHEM PHARMACOL. 2022 Aug;202:115117Read more>>
  • Guangning Kou. et al. Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Feng Wang. et al. Metformin reduces myogenic contracture and myofibrosis induced by rat knee joint immobilization via AMPK-mediated inhibition of TGF-_1/Smad signaling pathway. CONNECT TISSUE RES. 2022 Jun 2Read more>>
  • Wu Zhiqiang. et al. Jinlian Xiaodu Decoction Protects against Bleomycin-Induced Pulmonary Fibrosis in Rats. EVID-BASED COMPL ALT. 2022;2022:4206364Read more>>
  • Yifan Zhang. et al. MiR-208b/miR-21 Promotes the Progression of Cardiac Fibrosis Through the Activation of the TGF-_1/Smad-3 Signaling Pathway: An in vitro and in vivo Study. FRONT CARDIOVASC MED. 2022; 9: 924629Read more>>
  • Yu-jie Lu. et al. Ligustilide attenuates airway remodeling in COPD mice by covalently binding to MH2 domain of Smad3 in pulmonary epithelium, disrupting the Smad3-SARA interaction. PHYTOTHER RES. 2022 OcRead more>>
  • Lin Niu. et al. Magnolol alleviates pulmonary fibrosis inchronic obstructive pulmonary disease by targeting transient receptor potential vanilloid 4-ankyrin repeat domain. PHYTOTHER RES. 2023 JuRead more>>
  • Xiaochen Sun. et al. Study on the mechanism of stir-fried Fructus Tribuli in enhancing the essential hypertension treatment by an integrated spectrum-effect relationship-network pharmacology-metabolomics strategy. BIOMED PHARMACOTHER. 2023 Sep;165:11516Read more>>
  • Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
  • Yantong Guo. et al. Asiaticoside modulates human NK cell functional fate by mediating metabolic flexibility in the tumor microenvironment. PHYTOMEDICINE. 2024 Oct;133:155921Read more>>
VALIDATION IMAGES