bs-3447R-FITC [Conjugated Primary Antibody]
eNOS (Ser1177) Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: eNOS Ser1177

Modification Site: Ser1177

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4846

Swiss Prot: P29474

Source: KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser1177

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 133


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, I., et al. "Statins ameliorate pulmonary hypertension secondary to left ventricular dysfunction through the Rho‐kinase pathway and NADPH oxidase." Pediatric Pulmonology (2016).Read more>>
  • Yin, Yaling, et al. "Protective effect of Xin Mai Jia ultrafiltration extract on human umbilical vein endothelial cell injury induced by hydrogen peroxide and the effect on the NO‑cGMP signaling pathway." Experimental and therapeutic medicine 8.1 (2014): 38-48.Read more>>
  • Kim DY et al. SALM4 regulates angiogenic functions in endothelial cells through VEGFR2 phosphorylation at Tyr1175. FASEB J. 2019 Jun 6:fj201802516RR. Read more>>
  • Zhang Q et al. Sulfur dioxide induces vascular relaxation through PI3K/Akt/eNOS and NO/cGMP signaling pathways in rats. Hum Exp Toxicol. 2020 Mar 10:960327120911428.Read more>>
  • ?zel F et al. Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300. Read more>>
  • Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476Read more>>
  • Sol Guerra-Ojeda. et al. Cerium dioxide nanoparticles modulate antioxidant defences and change vascular response in the human saphenous vein. FREE RADICAL BIO MED. 2022 Nov;193:694Read more>>
  • Mengmeng Shen. et al. Erbu Zhuyu decoction improves endometrial angiogenesis via uterine natural killer cells and the PI3K/Akt/eNOS pathway a mouse model of embryo implantation dysfunction. AM J REPROD IMMUNOL. 2022 NovRead more>>
  • Luya Pu. et al. TXNRD1 knockdown inhibits the proliferation of endothelial cells subjected to oscillatory shear stress via activation of the endothelial nitric oxide synthase/apoptosis pathway. BBA-MOL CELL RES. 2023 Apr;1870:119436Read more>>
  • Min Luo. et al. Methyl protodioscin reduces c-Myc to ameliorate diabetes mellitus erectile dysfunction via downregulation of AKAP12. DIABETES RES CLIN PR. 2023 Nov;:111012Read more>>
  • Jian Liu. et al. Environmental dose of 16 priority-controlled PAHs induce endothelial dysfunction: An in vivo and in vitro study. SCI TOTAL ENVIRON. 2024 Apr;919:170711Read more>>
  • Sol Guerra-Ojeda. et al. Sodium valproate treatment reverses endothelial dysfunction in aorta from rabbits with acute myocardial infarction. EUR J PHARMACOL. 2024 May;970:176475Read more>>
  • Zhang Ning. et al. TMAO Impairs Mouse Aortic Vasodilation by Inhibiting TRPV4 Channels in Endothelial Cells. J CARDIOVASC TRANSL. 2024 Jul;:1-12Read more>>
VALIDATION IMAGES