bs-3446R [Primary Antibody]
Tau protein(Ser396) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Tau protein Ser396

Modification Site: Ser396

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4137

Swiss Prot: P10636

Source: KLH conjugated synthetic phosphopeptide derived from human Tau protein around the phosphorylation site of Ser396 [KT(p-S)T]

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 52/79


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cai, Zhiyou, Yong Yan, and Yonglong Wang. "Minocycline alleviates beta-amyloid protein and tau pathology via restraining neuroinflammation induced by diabetic metabolic disorder." Clinical Interventions in Aging 8 (2013): 1089-1095.Read more>>
  • Ku et al. Synergistic effects of particulate matter (PM2.5) and sulfur dioxide (SO2) on neurodegeneration via the microRNA-mediated regulation of tau phosphorylation. (2017) Toxicol.Res.(Camb). 6:7-16Read more>>
  • Ding J et al. The Effect of α-Synuclein and Tau in Methamphetamine Induced Neurotoxicity in Vivo and in Vitro. Toxicol Lett. 2020 Feb 1;319:213-224.Read more>>
  • Yihan Lu? et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.Read more>>
  • Xiangdong Meng. et al. Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer's disease by regulating multiple metabolic pathways. Mol Med Rep. 2021 May;23(5):1-13Read more>>
  • Singh Nidhi Anand K.. et al. Ellagic Acid Reverses Alterations in the Expression of AMPA Receptor and Its Scaffolding Proteins in the Cerebral Cortex and Memory Decline in STZ-sporadic Alzheimer s Disease Mouse Model. PSYCHOPHARMACOLOGY. 2024 Jun;:1-15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-Tau protein(Ser396) Polyclonal Antibody, Unconjugated (bs-3446R) at 1:200 followed by conjugation to the secondary antibody


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Tau protein(Ser396) Polyclonal Antibody, Unconjugated (bs-3446R) at 1:400 overnight at 4°C, DAB staining.