bs-3434R [Primary Antibody]
Syk (Tyr525 + Tyr526) Antibody
www.biossusa.com
[email protected]
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Host: Rabbit

Target Protein: Syk Tyr525 + Tyr526

Modification Site: Tyr525 + Tyr526

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6850

Swiss Prot: P43405

Source: KLH conjugated synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyr525/526

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 72


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Chicken
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
  • He S et al. Avian leukosis virus subgroup J induces B cell anergy mediated by Lyn inhibited BCR signal transduction. Vet Microbiol. 2020 Aug;247:108781.Read more>>
  • Chen, Xuejiao. et al. Targeting SYK of monocyte-derived macrophages regulates liver fibrosis via crosstalking with Erk/Hif1 and remodeling liver inflammatory environment. Cell Death Dis. 2021 Dec;12(12):1-11Read more>>
  • Xiangyu Liang. et al. Effects and potential mechanisms of Saposhnikovia divaricata (Turcz.) Schischk. On type I allergy and pseudoallergic reactions in vitro and in vivo. J ETHNOPHARMACOL. 2024 Jan;318:116942Read more>>
  • Xiao-Rong Huang. et al. Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1. AUTOPHAGY. 2024 29Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 mouse embryo lysates L2 mouse thymus lysates probed with Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 70kD. Observed band size:70kD.\n