bs-3425R-Biotin [Conjugated Primary Antibody]
Smad3 (Ser423 + Ser425) Antibody, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad3 Ser423 + Ser425

Specificity: These phosphorylation sites are homologous across the species listed.

Modification Site: Ser423 + Ser425

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4088

Swiss Prot: P84022

Source: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ghiabi, Pegah, et al. "Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche." Journal of translational medicine 13.1 (2015): 27.Read more>>
  • Shang, Peijin, et al. "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." Scientific Reports 6 (2016): 39809.Read more>>
  • Bai, Long, et al. "BAMBI promotes porcine granulosa cell steroidogenesis involving TGF-β signaling." Theriogenology (2017).Read more>>
  • Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).Read more>>
  • Shang et al. Telmisartan improves vascular remodeling through ameliorating prooxidant and profibrotic mechanisms in hypertension via the involvement of transforming growth factor-β1. (2017) Mol.Med.Re. 16:4537-4544Read more>>
  • Hu et al. Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition. (2016) PLoS.On. 11:e0153409Read more>>
  • Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
  • Wang et al. Epigallocatechin-3-gallate attenuates unilateral ureteral obstruction-induced renal interstitial fibrosis in mice. (2015) J.Histochem.Cytochem. 63:270-9Read more>>
  • Zhang W et al. Smad Anchor for Receptor Activation and Phospho-Smad3 Were Upregulated in Patients with Temporal Lobe Epilepsy.J Mol Neurosci. 2019 May;68(1):91-98.Read more>>
  • Wang JQ et al. Knockdown of microRNA-17-5p Enhances the Neuroprotective Effect of Act A/Smads Signal LoopAfter Ischemic Injury. Neurochem Res. 2019 May 15.Read more>>
  • Guo LP et al. Smad signaling coincides with epithelial-mesenchymal transition in a rat model of intrauterine adhesion. Am J Transl Res. 2019 Aug 15;11(8):4726-4737. eCollection 2019.Read more>>
  • Li L et al. Anti-fibrotic effect of melittin on TRIM47 expression in Human embryonic lung fibroblast through regulating TRIM47 pathway. Life Sci. 2020 Sep 1;256:117893.Read more>>
  • Siyu Li. et al. Exploring the liver fibrosis induced by deltamethrin exposure in quails and elucidating the protective mechanism of resveratrol. Ecotox Environ Safe. 2021 Jan;207:111501Read more>>
  • Naihua Hu. et al. Forsythiae Fructuse water extract attenuates liver fibrosis via TLR4/MyD88/NF-B and TGF-/smads signaling pathways. J Ethnopharmacol. 2020 Nov;262:113275Read more>>
  • Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13Read more>>
  • Haixia Li. et al. Aggravation of hepatic ischemia?reperfusion injury with increased inflammatory cell infiltration is associated with the TGF?/Smad3 signaling pathway. Mol Med Rep. 2021 Aug;24(2):1-1Read more>>
  • Xiaoyue Guan. et al. Gremlin aggravates periodontitis via activating the NF-_B signaling pathway. 2022 Jan 06Read more>>
  • Guangning Kou. et al. Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Feng Wang. et al. Metformin reduces myogenic contracture and myofibrosis induced by rat knee joint immobilization via AMPK-mediated inhibition of TGF-_1/Smad signaling pathway. CONNECT TISSUE RES. 2022 Jun 2Read more>>
  • Kim, Duk Ki. et al. PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141+ cancer-associated fibroblasts in pancreatic cancer. NAT COMMUN. 2022 Oct;13(1):1-19Read more>>
  • Ping Li. et al. Cyanocobalamin promotes muscle development through the TGF- signaling pathway. FOOD FUNCT. 2022 NovRead more>>
  • Xing Li. et al. Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGF-dependent squamous cell carcinoma lung metastasis. CANCER LETT. 2023 Aug;570:21633Read more>>
  • Genghua Chen. et al. Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation. CELL PROLIFERAT. 2023 Sep;:e13545Read more>>
  • Demircan Volkan. et al. Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis. SCI REP-UK. 2024 Mar;14(1):1-14Read more>>
  • Junfeng He. et al. Effect of the TGF-/BMP Signaling Pathway on the Proliferation of Yak Pulmonary Artery Smooth Muscle Cells under Hypoxic Conditions. ANIMALS. 2024 Jan;14(14):2072Read more>>
  • Ke Xu. et al. Polystyrene microplastics and di-2-ethylhexyl phthalate co-exposure: Implications for female reproductive health. Environmental Science and Ecotechnology. 2024 Nov;22:100471Read more>>
VALIDATION IMAGES