Host: Rabbit
Target Protein: TORC2 Ser171
Modification Site: Ser171
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 200186
Swiss Prot: Q53ET0
Source: KLH conjugated synthetic phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
Predicted Molecular Weight: 73
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Tian, Xiaohui, et al. "CRTC2 enhances HBV transcription and replication by inducing PGC1alpha expression." Virology Journal 11.1 (2014): 30.Read more>>
- Slocum, Stephen L., et al. "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." Archives of Biochemistry and Biophysics (2016).Read more>>
- Seok et al. Transcriptional regulation of autophagy by an FXR-CREB axis. (2014) Natur. 516:108-11Read more>>
- Cicerchi et al. Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids. (2014) FASEB.. 28:3339-50Read more>>
- Zhou TT et al. Small molecule IVQ, as a prodrug of gluconeogenesis inhibitor QVO, efficiently ameliorates glucose homeostasis in type 2 diabetic mice.Acta Pharmacol Sin. 2019 Mar 4. Read more>>
- Jizheng Wang. et al. Ceramide enhanced the hepatic glucagon response through regulation of CREB activity. CLIN NUTR. 2023 DecRead more>>
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SGK1 (Ser422) Polyclonal Antibody(bs-3415R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Spleen lysates probed with TORC2 (Ser171) Polyclonal Antibody, Unconjugated (bs-3415R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.