bs-3380R-Cy3 [Conjugated Primary Antibody]
Rb/P105 RB (Ser807 + Ser811) Antibody, Cy3 Conjugated
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Host: Rabbit

Target Protein: Rb/P105 RB Ser807 + Ser811

Modification Site: Ser807 + Ser811

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5925

Source: KLH conjugated synthetic phosphopeptide derived from human P105 RB around the phosphorylation site of Ser807/Ser811

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Nuclear MarkerRb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 102


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang, Xu, et al. "ICAM-1-Targeted Liposomes Loaded with Liver X Receptor Agonists Suppress PDGF-Induced Proliferation of Vascular Smooth Muscle Cells." Nanoscale Research Letters 12.1 (2017): 322.Read more>>
  • "Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470."Read more>>
  • Fei Min et al. Hepatoprotective effects of hydroxysafflor yellow A in D-galactose-treated aging mice. Eur J Pharmacol. 2020 Aug 15;881:173214.Read more>>