DATASHEET
Host:
Rabbit
Target Protein:
PLK1 Ser137
Specificity:
This phosphorylation site is homologous across the listed species.
Modification Site:
Ser137
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5347
Swiss Prot:
P53350
Source:
KLH conjugated synthetic phosphopeptide derived from human PLK1 around the phosphorylation site of Ser137
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Phosphorylates NEDD1. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylates both ECT2 and RACGAP1, and thereby stimulates their interaction that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2.
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-PLK1 (Ser137) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse large intestine lysates; probed with PLK1 (Ser137) Polyclonal Antibody, unconjugated (bs-3345R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human U-2 OS cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human MDA-MB-231 cell lysates probed with Phospho-PLK1 (Ser137) Polyclonal Antibody, Unconjugated (bs-3345R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.