bs-3332R-FITC [Conjugated Primary Antibody]
PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) antibody, FITC Conjugated
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Host: Rabbit

Target Protein: PI3 kinase p85 alpha + gamma Tyr467 + Tyr199

Modification Site: Tyr467 + Tyr199

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5295

Swiss Prot: P27986

Source: KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alphaTyr467

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 85/55


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).Read more>>
  • Jeong, Heesoo, Yanan Liu, and Hyun-Sook Kim. "Dried plum and chokeberry ameliorate d-galactose-induced aging in mice by regulation of Pl3k/Akt-mediated Nrf2 and Nf-kB pathways." Experimental Gerontology (2017).Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Zhang X et al. COX-2 promotes epithelial‑mesenchymal transition and migration in osteosarcoma MG‑63 cells via PI3K/AKT/NF‑κB signaling. Molecular Medicine REPORTS.2019 20: 3811-3819.Read more>>
  • Qiao J et al. Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway. OncoTargets and Therapy, 2019,12: 8569–8582. Read more>>
  • Yang RP et al. Pharmacological actions of neferine in the modulation of human platelet function. Eur J Pharmacol. 2019 Aug 21:172626. Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Jiang A et al. Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier. Int Immunopharmacol. 2019 Nov 8:105972.Read more>>
  • Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.Read more>>
  • Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2). Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964. Read more>>
  • Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094.Read more>>
  • Lu Xue. et al. A novel peptide relieves endothelial cell dysfunction in?preeclampsia by regulating the PI3K/mTOR/HIF1 pathway. Int J Mol Med. 2021 Jan;47(1):276-288Read more>>
  • Di Wu. et al. Alpinetin prevents inflammatory responses in OVA-induced allergic asthma through modulating PI3K/AKT/NF-B and HO-1 signaling pathways in mice. Int Immunopharmacol. 2020 Dec;89:107073Read more>>
  • Zhongbai Zhang. et al. Oxymatrine pretreatment protects H9c2 cardiomyocytes from hypoxia/reoxygenation injury by modulating the PI3K/Akt pathway. Exp Ther Med. 2021 Jun;21(6):1-12Read more>>
  • Li Ling. et al. A novel peptide promotes human trophoblast proliferation and migration through PI3K/Akt/mTOR signaling pathway. Ann Transl Med. 2021 Jun; 9(12): 981Read more>>
  • Jing Chen. et al. Apatinib enhances the anti-tumor effect of paclitaxel via the PI3K/p65/Bcl-xl pathway in triple-negative breast cancer. Ann Transl Med. 2021 Jun; 9(12): 1001Read more>>
  • Feng Liu. et al. CircRNA_0084927 promotes colorectal cancer progression by regulating miRNA-20b-3p/glutathione S-transferase mu 5 axis. World J Gastroentero. 2021 Sep 28; 27(36): 6064C6078Read more>>
  • Ma, Zhaochen. et al. Repurposing a clinically approved prescription Colquhounia root tablet to treat diabetic kidney disease via suppressing PI3K/AKT/NF-kB activation. Chin Med-Uk. 2022 Dec;17(1):1-13Read more>>
  • Huang, Wenjie. et al. The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters. CHIN MED-UK. 2022 Dec;17(1):1-18Read more>>
  • Yan LI. et al. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1 and VEGF via the PI3K/AKT and ERK1/2 signaling pathways. CHIN J NAT MEDICINES. 2022 Sep;20:679Read more>>
  • Wen-Ling Liao. et al. Inhibitory Effects of Ursolic Acid on the Stemness and Progression of Human Breast Cancer Cells by Modulating Argonaute-2. INT J MOL SCI. 2023 Jan;24(1):366Read more>>
  • Hanpeng Wu. et al. The release of FB1-induced heterophil extracellular traps in chicken is dependent on autophagy and glycolysis. POULTRY SCIENCE. 2023 Jan;:102511Read more>>
  • Kaifeng Liu. et al. PSMC4 promotes prostate carcinoma progression by regulating the CBX3CEGFR-PI3K-AKT-mTOR pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 JulRead more>>
  • Chen Jia. et al. A quality-comprehensive-evaluation-index-based model for evaluating traditional Chinese medicine quality. CHIN MED-UK. 2023 Dec;18(1):1-14Read more>>
  • Jin Yan-Bin. et al. Mechanism of action of icaritin on uterine corpus endometrial carcinoma based on network pharmacology and experimental evaluation. FRONT ONCOL. 2023 Jul;13Read more>>
  • Xiaoxiao Zhang. et al. Tanshinone A participates in the treatment of endometriosis by regulating adhesion, invasion, angiogenesis and inhibition of PI3K/Akt/mTOR signaling pathway. MOL MED REP. 2023 Nov;28(5):1-13Read more>>
  • Chaoyang Zhou. et al. Depleting ANTXR1 suppresses glioma growth via deactivating PI3K/AKT pathway. CELL CYCLE. 2023 Nov 16Read more>>
  • Feng Qiu. et al. The mechanism of Chebulae Fructus Immaturus promote diabetic wound healing based on network pharmacology and experimental verification. J ETHNOPHARMACOL. 2024 Mar;322:117579Read more>>
  • Zhang Tianyu. et al. Research on the Metabolic Regulation Mechanism of Yangyin Qingfei Decoction Plus in Severe Pneumonia Caused by Mycoplasma Pneumoniae in Mice. FRONT PHARMACOL. 2024 Mar;15Read more>>
  • Jinxing Yang. et al. KIF15 promotes the development and progression of chordoma via activating PI3K-AKT signalling pathway. HELIYON. 2024 Apr;:e29386Read more>>
  • Dating Pei. et al. Modulation of macrophage polarization by secondary cross-linked hyaluronan-dopamine hydrogels. INT J BIOL MACROMOL. 2024 Jun;270:132417Read more>>
  • Wang Jianan. et al. Association of attenuated leptin signaling pathways with impaired cardiac function under prolonged high-altitude hypoxia. SCI REP-UK. 2024 May;14(1):1-18Read more>>
  • Xin Bin. et al. circMTO1/miR-30c-5p/SOCS3 axis alleviates oral submucous fibrosis through inhibiting fibroblastmyofibroblast transition. J ORAL PATHOL MED. 2024 MayRead more>>
  • Wang Zhiyong. et al. EIF2S2 transcriptionally upregulates HIF1 to promote gastric cancer progression via activating PI3K/AKT/mTOR pathway. CARCINOGENESIS. 2024 JulRead more>>
VALIDATION IMAGES

Molt-4 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) antibody, FITC Conjugated（bs-3332R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).