DATASHEET
Swiss Prot:
N/A
Source:
Recombinant AmCyan-Tag protein, His protein is expressed in E.coli with His. It contains the amino acid sequence of 1-234/234.
Storage Buffer:
0.01M TBS(pH7.4)
Storage:
The product should be stored at -70°C or -20°C.
Background:
AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.