DATASHEET
Host:
Rabbit
Target Protein:
Mst1 Thr183 + Mst2 Thr180
Modification Site:
Thr 183/Thr180
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
6789, 6788
Swiss Prot:
Q13043, Q13188
Source:
KLH conjugated synthetic phosphopeptide derived from human Mst1 around the phosphorylation site of Thr183
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Protein which catalyzes the phosphorylation of serine or threonine residues on target proteins by using ATP as phosphate donor. Such phosphorylation may cause changes in the function of the target protein. Protein kinases share a conserved catalytic core common to both serine/ threonine and tyrosine protein kinases.
Conjugation:
FITC
Excitation/ Emission:
494nm/518nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
19/37/56
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao R et al. PM2.5-associated nitro-PAH exposure promotes tumor cell metastasis through Hippo-YAPmediated transcriptional regulation. Sci Total Environ. 2019 Aug 15;678:611-617. Read more>>
- Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
- Jinjing Jia. et al. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein. 2021 May 07Read more>>
- Pin-Shi Ni. et al. Indirect regulation of HIPPO pathway by miRNA mediates high-intensity intermittent exercise to ameliorate aging skeletal muscle function. SCAND J MED SCI SPOR. 2023 FebRead more>>
- Shouying Xu. et al. ARID1A restrains EMT and stemness of ovarian cancer cells through the Hippo pathway. INT J ONCOL. 2024 Aug;65(2):1-11Read more>>
VALIDATION IMAGES
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃. Cells were then stained with Mst1(Thr183)/Mst2(Thr180) Antibody(bs-3294R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).