bs-3272R-Cy3

DATASHEET

Host: Rabbit

Target Protein: c-Met Tyr1234 + Tyr1235

Specificity: These phosphorylation sites are homologous to Tyr1232 + Ty1233 in Mouse and Tyr1235 + Tyr1236 in Rat. Due to the highly conserved nature of this domain, there is a chance that this antibody will react with MSP receptors in Mouse and, less likely, in Human when phosphorylated at the the sites of Ser1238 and Ser1239 in Human, Ser1215 and Ser1216 in Mouse. No phosphorylation information is currently available Rat.

Modification Site: Tyr1234 + Tyr1235

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4233

Swiss Prot: P08581

Source: KLH conjugated synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyr1234/1235

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 153

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.