bs-3237R-Cy3 [Conjugated Primary Antibody]
IKK alpha / beta (S176 / S180) Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IKK alpha/beta Ser176 / Ser180

Specificity: These phosphorylation sites are homologous across the listed species.

Modification Site: Ser176 + Ser180

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1147

Swiss Prot: O15111

Source: KLH conjugated synthetic phosphopeptide derived from human IKK alpha/beta around the phosphorylation site of Ser176/Ser180

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 85/87


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Hongyu, et al. "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway."Life Sciences (2016).Read more>>
  • Ma, Zhihong, et al. "Paeoniflorin alleviates non-alcoholic steatohepatitis in rats: Involvement with the ROCK/NF-κB pathway." International Immunopharmacology 38 (2016): 377-384.Read more>>
  • Gao, Wenyan, et al. "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." Acta Histochemica (2016).Read more>>
  • obae Kimet al. B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues. Int J Mol Sci. 2020 Jul 20;21(14):5121.Read more>>
  • Chung-Jen Chiang. et al. Diabetes-induced cardiomyopathy is ameliorated by heat-killed Lactobacillus reuteri GMNL-263 in diabetic rats via the repression of the toll-like receptor 4 pathway. 2021 Feb 08Read more>>
  • Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
  • Dan Li. et al. Piperazine ferulate attenuates gentamicin-induced acute kidney injury via the NF-_B/NLRP3 pathway. Phytomedicine. 2022 Feb;:154021Read more>>
  • Ya-Nan Gao. et al. Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. SCI TOTAL ENVIRON. 2022 Sep;:158777Read more>>
  • Kuangyang Yang. et al. Identification of Andrographolide as a novel FABP4 inhibitor for osteoarthritis treatment. PHYTOMEDICINE. 2023 Sep;118:154939Read more>>
  • Ting Li. et al. The Effect of Bergenin on Isonicotinic Acid Hydrazide and Rifampicin-Induced Liver Injury Revealed by RNA Sequencing. MOLECULES. 2023 Jan;28(14):5496Read more>>
VALIDATION IMAGES