bs-3201R-Biotin

DATASHEET

Host: Rabbit

Target Protein: IRS1 Ser636 + Ser639

Specificity: This phosphorylation site is homologous to Ser632 + ser635 in Mouse and Rat IRS1. This protein is known to be highly conserved in this sequences region, thus , this antibody may react with IRS2 when phosphorylated at Ser679 + Ser682 in Human , Ser675 + Ser678 in Mouse, and Ser676 +Ser679 in Rat based on homology.

Modification Site: Ser636 + Ser639

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3667

Swiss Prot: P35568

Source: KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639 [PM(p-S)PK(p-S)VS]

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 132

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.