Host: Rabbit
Target Protein: IRS1 Ser636 + Ser639
Specificity: This phosphorylation site is homologous to Ser632 + ser635 in Mouse and Rat IRS1. This protein is known to be highly conserved in this sequences region, thus , this antibody may react with IRS2 when phosphorylated at Ser679 + Ser682 in Human , Ser675 + Ser678 in Mouse, and Ser676 +Ser679 in Rat based on homology.
Modification Site: Ser636 + Ser639
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3667
Swiss Prot: P35568
Source: KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639 [PM(p-S)PK(p-S)VS]
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 132
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti phospho-IRS1(Ser636/639) Polyclonal Antibody, Unconjugated (bs-3201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRS1(Ser636 + Ser639) Polyclonal Antibody, Unconjugated (bs-3201R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRS1(Ser636 + Ser639) Polyclonal Antibody, Unconjugated (bs-3201R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRS1(Ser636 + Ser639) Polyclonal Antibody, Unconjugated (bs-3201R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IRS1(Ser636 + Ser639) Polyclonal Antibody, Unconjugated (bs-3201R) at 1:200 overnight at 4°C, DAB staining.