bs-3196R
[Primary Antibody]
IRF7 (Ser471 + Ser472) Antibody
DATASHEET
Host: Rabbit
Target Protein: IRF7 Ser471 + Ser472
Modification Site: Ser471 + Ser472
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3665
Swiss Prot: Q92985
Source: KLH conjugated synthetic phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 54
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Han, Young Woo, et al. " Distinct Dictation of Japanese Encephalitis Virus-Induced Neuroinflammation and Lethality via Triggering TLR3 and TLR4 Signal Pathways." PLoS Pathogens 10(9) (2014): e103882.Read more>>
- Le Bel, Manon, and Jean Gosselin. "Leukotriene B 4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." PloS one 10.10 (2015): e0139856.Read more>>
- Egarnes, Benoit, Marie-Renée Blanchet, and Jean Gosselin. "Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice." PLOS ONE 12.10 (2017): e0186639.Read more>>
- Menasria et al. Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection. (2013) J.Virol. 87:7301-8Read more>>
- Egarnes et al. Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice. (2017) PLoS.One. 12:e0186639Read more>>
- Peng K. et al. Transcriptome Analyses of Senecavirus A-Infected PK-15 Cells: RIG-I and IRF7 Are the Important Factors in Inducing Type III Interferons.. Front Microbiol. 2022 Mar;13:846343-846343Read more>>
- Anette Christ. et al. Low Density Lipoprotein Exposure of Plasmacytoid Dendritic Cells Blunts Toll-like Receptor 7/9 Signaling via NUR77. BIOMEDICINES. 2022 May;10(5):1152Read more>>
- HuiDan Deng. et al. Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication. FRONT IMMUNOL. 2022; 13: 955671Read more>>
- Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN- signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044Read more>>
- Dadi Peng. et al. PPM1G regulates hepatic ischemia/reperfusion injury through STING-mediated inflammatory pathways in macrophages. IMMUN INFLAMM DIS. 2024 Feb;12(2):e1189Read more>>
- Zhou Shengyang. et al. The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinsons disease. NEURAL REGEN RES. 2024 Jun;:10.4103/NRR.NRRRead more>>
VALIDATION IMAGES
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti Phospho-IRF7 (Ser471/472) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 54kD. Observed band size: 54kD
Formalin-fixed and paraffin embedded mouse pancreas tissue labeled with Anti-Phospho-IRF7 (Ser471/472) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spleen tissue labeled with Anti-Phospho-IRF7 (Ser471/472) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse uterus lysates; Lane 2: Mouse large intestine lysates probed with IRF7 (Ser471 + Ser472) Polyclonal Antibody, unconjugated (bs-3196R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRF7 (Ser471 + Ser472) Antibody(bs-3196R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Blood cell lysates; Lane 4: Mouse Lung tissue lysates ; Lane 5: Rat Spleen tissue lysates ; Lane 6: Rat Liver tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human Raji cell lysates probed with Phospho-IRF7 (Ser471 + Ser472) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.