bs-3195R-HRP [Conjugated Primary Antibody]
IRF3 (Ser396) Polyclonal Antibody, HRP Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRF3 Ser396

Modification Site: Ser396

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3661

Swiss Prot: Q14653

Source: KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Monkey

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ichikawa, Tomohiro, et al. "TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts." The Journal of Immunology (2014): 1302919.Read more>>
  • Le Bel, Manon, and Jean Gosselin. "Leukotriene B 4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." PloS one 10.10 (2015): e0139856.Read more>>
  • Ando, Makoto, et al. "Poly (I: C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries." Free Radical Biology and Medicine (2017).Read more>>
  • Egarnes, Benoit, Marie-Renée Blanchet, and Jean Gosselin. "Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice." PLOS ONE 12.10 (2017): e0186639.Read more>>
  • Menasria et al. Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection. (2013) J.Virol. 87:7301-8Read more>>
  • Egarnes et al. Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice. (2017) PLoS.One. 12:e0186639Read more>>
  • Wan B et al. Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction. Int J Biol Macromol. 2019 Jul 5;138:198-206. Read more>>
  • H.Q. Hu. et al. The STING-IRF3 pathway is involved in lipotoxic injury of pancreatic cells in type 2 diabetes. Mol Cell Endocrinol. 2020 Dec;518:11089Read more>>
  • Ting-Jing Shen. et al. Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation. J CLIN INVEST. 2022 Sep;():142805Read more>>
  • Zhai Jinxia. et al. Gut Microbiota Metabolites Mediate Bax to Reduce Neuronal Apoptosis via cGAS/STING Axis in Epilepsy. MOL NEUROBIOL. 2023 Aug;:1-16Read more>>
  • Gallage Suchira. et al. Ribosomal S6 kinase 1 regulates inflammaging via the senescence secretome. Nature Aging. 2024 Aug;:1-18Read more>>
  • Loretah Chibaya. et al. Nanoparticle delivery of innate immune agonists combined with senescence-inducing agents promotes T cell control of pancreatic cancer. SCI TRANSL MED. 2024 Aug;16(762)Read more>>
VALIDATION IMAGES