DATASHEET
Host:
Rabbit
Target Protein:
Histone H2A.X Ser140
Specificity:
This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.
Modification Site:
Ser140
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3014
Swiss Prot:
P16104
Source:
KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Conjugation:
FITC
Excitation/ Emission:
494nm/518nm
Size:
100ul
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
16
Human
Others
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Song, Xiufang, et al. "An unpredicted downregulation of RAD51 suggests genome instability induced by tetrachlorobenzoquinone." Chemical Research in Toxicology (2016).Read more>>
- Shi et al. Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress. (2017) Oncotarget. 8:45981-45993Read more>>
- Wang L et al. Antimalarial Dihydroartemisinin Triggers Autophagy within HeLa Cells of Human Cervical Cancer through Bcl-2 Phosphorylation at Ser70. (2018) Phytomedicine. 7113(18)30503-8Read more>>
- Chen X et al. Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells.(2019)Oncol Rep. Feb;41(2):765-778.Read more>>
- Zhang Q et al. Photoactivatable Prodrug-Backboned Polymeric Nanoparticles for Efficient Light-Controlled Gene Delivery and Synergistic Treatment of Platinum-Resistant Ovarian Cancer. Nano Lett. 2020 Apr 9. Read more>>
- Liu Yahong. et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev. 2021;2021:6683836Read more>>
- Sun Yuhuan. et al. Near-infrared-traceable DNA nano-hydrolase: specific eradication of telomeric G-overhang in vivo. Nucleic Acids Res. 2020 Sep;48(17):9986-9994Read more>>
- Donglin Xia. et al. AuCHemoglobin Loaded Platelet Alleviating Tumor Hypoxia and Enhancing the Radiotherapy Effect with Low-Dose X-ray. Acs Nano. 2020;14(11):15654C15668Read more>>
- Li na Wang. et al. Fighting against Drug\Resistant Tumors by Inhibition of -Glutamyl Transferase with Supramolecular Platinum Prodrug Nano-Assemblies. 2021 May 06Read more>>
- Yuan SJ. et al. Conjugation with nanodiamonds via hydrazone bond fundamentally alters intracellular distribution and activity of doxorubicin.. Int J Pharmaceut. 2021 Jul;606:120872-120872Read more>>
- Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
- Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
- Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
- Liu-Gen Li. et al. Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage. FRONT PHARMACOL. 2022; 13: 949835Read more>>
- Siyun Lei. et al. PARP inhibitors intervene DNA damage repair for the enhancement of tumor photodynamic therapy. PHOTODIAGN PHOTODYN. 2022 Aug;:103058Read more>>
- Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
- Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
- Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
- Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
- Zi-Yi Yang. et al. Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy. PHOTODIAGN PHOTODYN. 2023 Nov;:103917Read more>>
- Xu Hua-Zhen. et al. Anti-lung cancer synergy of low-dose doxorubicin and PD-L1 blocker co-delivered via mild photothermia-responsive black phosphorus. DRUG DELIV TRANSL RE. 2024 Apr;:1-22Read more>>
- Hui Yuan-Jian. et al. B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer. SCI REP-UK. 2024 Apr;14(1):1-15Read more>>
VALIDATION IMAGES
U2-OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H2A.X (Ser140) Antibody(bs-3185R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).