bs-3159R [Primary Antibody]
FAK (Tyr397) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FAK Tyr397

Specificity: This phosphorylation site is homologous to that of Tyr428 in Mouse and Tyr397 in Rat.

Modification Site: Tyr397

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5747

Swiss Prot: Q05397

Source: KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 116


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yunes-Medina, Laura, et al. "Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability." Molecular and Cellular Neuroscience (2017).Read more>>
  • Zhiguo Ji. et al. EGFR/FAK and c\Src signalling pathways mediate the internalisation of Staphylococcus aureus by osteoblasts. Cell Microbiol. 2020 Oct;22(10):e1324Read more>>
  • Li-Hua Muet al. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf. J Nat Med. 2020 Sep;74(4):732-740.Read more>>
  • Hu Li. et al. Injectable recombinant human collagen-derived material with high cell adhesion activity limits adverse remodelling and improves pelvic floor function in pelvic floor dysfunction rats. Mat Sci Eng C-Mater. 2022 Feb;:112715Read more>>
  • Jian Song. et al. The dual FAK-HDAC inhibitor MY-1259 displays potent activities in gastric cancers in vitro and in vivo. BIOORG CHEM. 2023 Feb;131:106328Read more>>
  • Yingmin Liu. et al. LOXL2 promotes tumor proliferation and metastasis by FAK/Src signaling in esophageal squamous cell carcinoma. ELECTRON J BIOTECHN. 2023 ApRead more>>
  • Seong-Beom Han. et al. Selective Suppression of Integrin-Ligand Binding by Single Molecular Tension Probes Mediates Directional Cell Migration. ADV SCI. 2024 Feb;:2306497Read more>>
  • Wenzhao Wang. et al. 3D printing of personalized magnesium composite bone tissue engineering scaffold for bone and angiogenesis regeneration. CHEM ENG J. 2024 Mar;484:149444Read more>>
  • Dating Pei. et al. Modulation of macrophage polarization by secondary cross-linked hyaluronan-dopamine hydrogels. INT J BIOL MACROMOL. 2024 Jun;270:132417Read more>>
  • Xin Bin. et al. circMTO1/miR-30c-5p/SOCS3 axis alleviates oral submucous fibrosis through inhibiting fibroblastmyofibroblast transition. J ORAL PATHOL MED. 2024 MayRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 mouse intestine lysate L2 rat lung lysates probed with Anti Phospho-FAK (Tyr397)Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 119kD. Observed band size: 55kD, 60kD, and 119kD


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Rat Adrenal gland lysates; Lane 6: Rat Testis lysates; Lane 7: Human 293T cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human HUVEC cell lysates; Lane 10: Human MDA-MB-231 cell lysates; Lane 11: Human A549 cell lysates probed with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAK (Tyr397) Polyclonal Antibody, Unconjugated (bs-3159R) at 1:200 overnight at 4°C, DAB staining.