bs-3154R [Primary Antibody]
c-Fos (Thr325) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: c-Fos Thr325

Specificity: This phosphorylation site is homologous to Thr325 in Mouse and Rat.

Modification Site: Thr325

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2353

Swiss Prot: P01100

Source: KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 41


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Sheep
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tavares, Raquel, and Sushil Kumar Pathak. "Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways." Frontiers in Cellular and Infection Microbiology 7 (2017): 58.Read more>>
  • Zheng N et al. Chlamydia pneumoniae infection promotes vascular smooth muscle cell migration via c-Fos/interleukin-17C signaling. International Journal of Medical Microbiology,2019, 151340. Read more>>
  • Mei Ha. et al. PKC mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism. Sci Total Environ. 2021 Feb;757:143727Read more>>
  • Ya-Ru Huang. et al. ArhGAP11A mediates amyloid- generation and neuropathology in an Alzheimer's disease-like mouse model. CELL REP. 2023 Jun 9;42(6):112624Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Phospho-c-Fos (Thr325)Polyclonal Antibody, Unconjugated (bs-3154R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Human Hela cells probed with c-Fos (Thr325) Polyclonal Antibody, Alexa Fluor 488 conjugated (bs-3154R-A488) (green) at 1:100 for 30 minutes compared to unstained cells (blue) and isotype control (orange).