bs-3140R [Primary Antibody]
FoxO3a (Ser253) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FoxO3a Ser253

Specificity: This phosphorylation site is homologous to Ser252 in Mouse and Rat.

Modification Site: Ser253

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2309

Swiss Prot: O43524

Source: KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Morales, María Gabriela, et al. "Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy via the Mas receptor." Disease Models and Mechanisms(2016): dmm-023390.Read more>>
  • Li Z et al. LncIRS1 controls muscle atrophy via sponging miR‐15 family to activate IGF1‐PI3K/AKT pathway. J Cachexia Sarcopenia Muscle. 2019 Jan 30. Read more>>
  • Shang Y et al. Over\expressed MST1 impaired spatial memory via disturbing neural oscillation patterns in mice. Genes Brain Behav. 2020 Jul;19(6):e12678.Read more>>
  • Shuang-Yan Chang. et al. miR-320 regulates myogenesis by targeting growth factor receptor-bound protein-2 and ameliorates myotubes atrophy. INT J BIOCHEM CELL B. 2022 Jun;147:106212Read more>>
  • Orozco-Aguilar Josu. et al. Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic flux. BIOL RES. 2023 Dec;56(1):1-19Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FoxO3a (Ser253) Polyclonal Antibody, Unconjugated (bs-3140R) at 1:400 overnight at 4°C, DAB staining.