bs-3073R [Primary Antibody]
HDAC4(Ser246)/HDAC5(Ser259)/HDAC7(Ser155) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HDAC4 Ser246/HDAC5 Ser259/HDAC7 Ser155

Modification Site: Ser155

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9759

Source: KLH conjugated synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 119


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC4(Ser246)/HDAC5(Ser259)/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC4(Ser246)/HDAC5(Ser259)/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with HDAC4(Ser246)/HDAC5(Ser259)/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.