Host: Rabbit
Target Protein: CEBP alpha Thr226
Specificity: This phosphorylated site is homologous to that of Thr222 in Mouse and Rat.
Modification Site: Thr226
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1050
Swiss Prot: P49715
Source: KLH conjugated synthetic phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr226
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 38
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Pig
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: mouse mouse lysates Lane 2: mouse heart lysates probed with Anti Phospho-CEBP alpha (Thr222/226)Polyclonal Antibody, Unconjugated (bs-3061R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 45kD. Observed band size: 45kD.
Antigen: bs-3061P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450
Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Phospho-CEBP alpha (Thr222 + Thr226) Polyclonal Antibody, Unconjugated (bs-3061R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.