bs-2993R-BF488 [Conjugated Primary Antibody]
IRF3 Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRF3

Immunogen Range: 51-150/427


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3661

Swiss Prot: Q14653

Source: KLH conjugated synthetic peptide derived from human IRF3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Human
Mouse
Rat
Bovine
Monkey

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fan, Wen-Jie, et al. "NF-κB is involved in the LPS-mediated proliferation and apoptosis of MAC-T epithelial cells as part of the subacute ruminal acidosis response in cows." Biotechnology Letters (2016): 1-11.Read more>>
  • Wan B et al. Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction. Int J Biol Macromol. 2019 Jul 5;138:198-206. Read more>>
  • Shenjie Zhong. et al. Bmp8a is an essential positive regulator of antiviral immunity in zebrafish. Commun Biol. 2021 Mar;4(1):1-15Read more>>
  • Wang Dong. et al. Classical swine fever virus NS4B protein interacts with MAVS and inhibits IL-8 expression in PAMs. Virus Res. 2021 Nov;:198622Read more>>
  • Xiaobao Gong. et al. Synthesis and anti-inflammatory activity of paeonol derivatives with etherized aryl urea by regulating TLR4/MyD88 signaling pathway in RAW264.7 cell. BIOORG CHEM. 2022 Oct;127:105939Read more>>
  • Wang Shang. et al. Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimers disease in APP/PS1 mouse. ALZHEIMERS RES THER. 2023 Dec;15(1):1-17Read more>>
  • Zhai Jinxia. et al. Gut Microbiota Metabolites Mediate Bax to Reduce Neuronal Apoptosis via cGAS/STING Axis in Epilepsy. MOL NEUROBIOL. 2023 Aug;:1-16Read more>>
  • Lihui Chen. et al. Bmp4 in Zebrafish Enhances Antiviral Innate Immunity through p38 MAPK (Mitogen-Activated Protein Kinases) Pathway. INT J MOL SCI. 2023 Jan;24(19):14444Read more>>
  • Hong-Fei Wang. et al. Jujuboside B alleviates acetaminophen-induced hepatotoxicity in mice by regulating Nrf2-STING signaling pathway. ECOTOX ENVIRON SAFE. 2024 Jan;269:11581Read more>>
VALIDATION IMAGES