bs-2973R [Primary Antibody]
SMAD5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMAD5

Specificity: The sequence used to produce this antibody is conserved in Smad 1, 2, 3 and 9 and expected to cross react due to sequence homology.

Immunogen Range: 360-410/465


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4090

Swiss Prot: Q99717

Source: KLH conjugated synthetic peptide derived from human SMAD5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:200-1000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
ICC/IF(ICC/IF=1:100)

Predicted Molecular Weight: 52


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yu M et al. BMP4 mutations in tooth agenesis and low bone mass. Arch Oral Biol. 2019 Jul;103:40-46.Read more>>
VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SMAD1 + SMAD5 Polyclonal Antibody, Unconjugated (bs-2973R) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.