bs-2972R-PE [Conjugated Primary Antibody]
HES1 Polyclonal Antibody, PE Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HES1

Immunogen Range: 41-150/280


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3280

Swiss Prot: Q14469

Source: KLH conjugated synthetic peptide derived from human HES1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 30


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Fei, et al. "Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer." Scientific Reports 4 (2014).Read more>>
  • Shi, Yan, et al. "Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately." Stem cell research & therapy 6.1 (2015): 1-13.Read more>>
  • Gao et al. Hes1 promotes cell proliferation and migration by activating Bmi-1 and PTEN/Akt/GSK3β pathway in human colon cancer. (2015) Oncotarge. 6:38667-80Read more>>
  • Wang et al. Hes1 triggers epithelial-mesenchymal transition (EMT)-like cellular marker alterations and promotes invasion and metastasis of nasopharyngeal carcinoma by activating the PTEN/AKT pathway. (2015) Oncotarge. 6:36713-30Read more>>
  • Cho-Won Kim et al. Inhibitory effects of cigarette smoke extracts on neural differentiation of Mouseembryonic stem cells. Reprod Toxicol. 2020 Aug;95:75-85.Read more>>
  • Chong Yin. et al. MACF1 alleviates aging-related osteoporosis via HES1. 2021 Jun 15Read more>>
VALIDATION IMAGES