Host: Rabbit
Target Protein: Maltose Binding Protein/MBP
Immunogen Range: 1-100/396
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 948538
Swiss Prot: P0AEX9
Source: KLH conjugated synthetic peptide derived from E. coli strain 12 Maltose Binding Protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Tags
Others
(Escherichia coli strain K12)
For research use only. Not intended for diagnostic or therapeutic use.
Antigen: bs-2967P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm.
Lane 1: MBP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33131P) probed with Maltose Binding Protein/MBP Polyclonal Antibody, Unconjugated (bs-2967R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MBP-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41233P) probed with MBP tag Polyclonal Antibody, Unconjugated (bs-2967R) at 1:200000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
100 ng rhC-jun-His-MBP protein (bs-42050P) per lane probed with MBP tag polyclonal antibody respectively, unconjugated (bs-2967R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.