bs-2957R
[Primary Antibody]
Alpha-ENaC Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Alpha-ENaC
Immunogen Range: 201-300/669
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6337
Swiss Prot: P37088
Source: KLH conjugated synthetic peptide derived from human Alpha-ENaC
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 76
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yamamoto, Seiji, et al. "Mineralocorticoid receptor stimulation induces urinary storage dysfunction via upregulation of epithelial sodium channel expression in the rat urinary bladder epithelium." Journal of Pharmacological Sciences (2016).Read more>>
- Yamamoto, Seiji, et al. "High salt loading induces urinary storage dysfunction via upregulation of epithelial sodium channel alpha in the bladder epithelium in Dahl salt-sensitive rats." Journal of Pharmacological Sciences (2017).Read more>>
- Scortegagna et al. The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion. (2017) JCI.Insight. 2 Read more>>
- Zhen-zhenChen et al. Bu Yang Huan Wu Decoction prevents reperfusion injury following ischemic stroke in rats via inhibition of HIF-1 α, VEGF and promotion β-ENaC expression (2018)Journal of Ethnopharmacology Sep 13;228:70-81.Read more>>
- Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51C6Read more>>
- Xiu Fei. et al. Aldosterone alleviates lipopolysaccharide-induced acute lung injury by regulating epithelial sodium channel through PI3K/Akt/SGK1 signaling pathway. Mol Cell Probe. 2021 Mar;:101709Read more>>
- Qian, ZhiZhi. et al. Protein nanoparticle-induced osmotic pressure gradients modify pulmonary edema through hyperpermeability in acute respiratory distress syndrome. J NANOBIOTECHNOL. 2022 Dec;20(1):1-21Read more>>
- enedikt Fels. et al. Effects of Chronic Kidney Disease on Nanomechanics of the Endothelial Glycocalyx Are Mediated by the Mineralocorticoid Receptor. INT J MOL SCI. 2022 Jan;23(18):10659Read more>>
- Nami Obayashi. et al. Effect of irinotecan administration on amiloride-sensitive sodium taste responses in mice. EUR J ORAL SCI. 2023 Feb;131(2):e12922Read more>>
- Zheng Qian. et al. Inactivation of epithelial sodium ion channel molecules serves as effective diagnostic biomarkers in clear cell renal cell carcinoma. GENES GENOM. 2023 May;:1-12Read more>>
- Yu-qiong He. et al. Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway. INT IMMUNOPHARMACOL. 2023 Dec;125:111161Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Rat kidney lysate at 40µg; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4°C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.
Mouse kidney lysate at 40µg; Probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 overnight at 4°C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Large intestine lysates probed with Alpha-ENaC Polyclonal Antibody, Unconjugated (bs-2957R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with SCNN1A polyclonal antibody, unconjugated (bs-2957R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.