bs-2951R
[Primary Antibody]
SQSTM1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: SQSTM1
Immunogen Range: 51-150/440
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8878
Swiss Prot: Q13501
Source: KLH conjugated synthetic peptide derived from human SQSTM1/Ubiquitin-binding protein p62
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 60
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xia, Xj, et al. "Autophagy mediated by arginine depletion activation of the nutrient sensor GCN2 contributes to interferon-γ-induced malignant transformation of primary bovine mammary epithelial cells." Cell Death Discovery 2 (2016).Read more>>
- Kuo, Hsiao-Mei, et al. "Altered Mitochondrial Dynamics and Response to Insulin in Cybrid Cells Harboring a Diabetes-susceptible Mitochondrial DNA Haplogroup." Free Radical Biology and Medicine (2016).Read more>>
- Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54.Read more>>
- Wang et al. Blocking autophagic flux enhances matrine-induced apoptosis in human hepatoma cells. (2013) Int.J.Mol.Sc. 14:23212-30Read more>>
- Yang and Yao JNK-Bcl-2/Bcl-xL-Bax/Bak Pathway Mediates the Crosstalk between Matrine-Induced Autophagy and Apoptosis via Interplay with Beclin 1. (2015) Int.J.Mol.Sci. 16:25744-58Read more>>
- Yin X et al. Lithium facilitates removal of misfolded proteins and attenuated faulty interaction between mutant SOD1 and p-CREB (Ser133) through enhanced autophagy in mutant hSOD1G93A transfected neuronal cell lines. Mol Biol Rep. 2019 Sep 16. Read more>>
- Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. Read more>>
- Wang B et al. Resolvin D1 Resolve Inflammation in Experimental Acute Pancreatitis by Restoring Autophagic Flux.Dig Dis Sci. 2018 Dec;63(12):3359-3366. Read more>>
- Kong Q et al. Autophagy inhibits TLR4\mediated invasiveness of oral cancer cells via the NF\B pathway. Oral Dis. 2020 Apr 14.Read more>>
- Jiachen Lv. et al. Alcohol Aggravates Acute Pancreatitis by Impairing Autophagic Flux Through Activation of AMPK Signaling Pathway. 2021 Feb 08Read more>>
- Sheng-Tao Ling. et al. Hydroxychloroquine Blocks Autophagy and Promotes Apoptosis of the Prostate after Castration in Rats. Urol Int. 2020;104(11-12):968-974Read more>>
- Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625Read more>>
- Gustavo Lopez-Toledo. et al. Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimers Disease Pathology, Constitute a Potential Object for Early Diagnosis. FRONT AGING NEUROSCI. 2022; 14: 921573Read more>>
- Zhang, Kai. et al. Long-Term Copper Exposure Induced Excessive Autophagy of the Porcine Spleen. BIOL TRACE ELEM RES. 2022 Jul;:1-9Read more>>
- Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549Read more>>
- Liu, Zhan. et al. Kaemperfol Protects Dopaminergic Neurons by Promoting mTOR-Mediated Autophagy in Parkinsons Disease Models. NEUROCHEM RES. 2022 Dec;:1-17Read more>>
- Shu-wen Xu. et al. Cigarette smoke extract-induced inflammatory response via inhibition of the TFEB-mediated autophagy in NR8383 cells. EXP LUNG RES. 2023 Jan 13Read more>>
- Xiudan Han. et al. Placental Mesenchymal Stem Cells Alleviate Podocyte Injury in Diabetic Kidney Disease by Modulating Mitophagy via the SIRT1-PGC-1alpha-TFAM Pathway. INT J MOL SCI. 2023 Jan;24(5):4696Read more>>
- Tengyun Yang. et al. MicroRNA-146a-5p alleviates the pathogenesis of osteoarthritis by inhibiting SDF-1/CXCR4-induced chondrocyte autophagy. INT IMMUNOPHARMACOL. 2023 Apr;117:109938Read more>>
- Muhammad Abid Hayat. et al. Enhanced Autophagy in Damaged Laminar Tissue of Acute Laminitis Induced by Oligofructose Overloading in Dairy Cows. ANIMALS. 2023 Jan;13(15):2478Read more>>
- Wenying Sun. et al. BPA and low-Se exacerbate apoptosis and mitophagy in chicken pancreatic cells by regulating the PTEN/PI3K/AKT/mTOR pathway. J ADV RES. 2024 FebRead more>>
- Huan Deng. et al. Low selenium and T-2 toxin may be involved in the pathogenesis of Kashin-Beck disease by affecting AMPK/mTOR/ULK1 pathway mediated autophagy. ECOTOX ENVIRON SAFE. 2024 Jul;279:116503Read more>>
- Chunhua Zhu. et al. Hypervirulent fowl adenovirus serotype 4 elicits early innate immune response and promotes virus-induced cellular autophagy in the spleen. POULTRY SCI. 2024 May;:103831Read more>>
- Yi Shang. et al. CircIQCH Contributes to the Progression of Breast Cancer by Elevating NFIB Through Decoying miR-139-5p. ENVIRON TOXICOL. 2024 JuRead more>>
- Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921Read more>>
- Yaning Biao. et al. Wulingsan Alleviates MAFLD by Activating Autophagy via Regulating the AMPK/mTOR/ULK1 Signaling Pathway. CAN J GASTROENTEROL. 2024 Jul;2024(1):9777866Read more>>
- Jinfeng Shang. et al. Naoxintong capsule accelerates mitophagy in cerebral ischemia-reperfusion injury via TP53/PINK1/PRKN pathway based on network pharmacology analysis and experimental validation. J ETHNOPHARMACOL. 2024 Aug;:118721Read more>>
- Lihong Liang. et al. S100A9-TLR4 axis aggravates dry eye through the blockage of autophagy. EXP EYE RES. 2024 Aug;:110052Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates, L2 rat brain lysates probed : Anti-GDF9 (bs-2951R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 48kDa and 62kD
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4°C, DAB staining.