bs-2946R [Primary Antibody]
HAS1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HAS1

Immunogen Range: 501-578/578


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3036

Swiss Prot: Q92839

Source: KLH conjugated synthetic peptide derived from human Hyaluronan synthase 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction. Also able to catalyze the synthesis of chito-oligosaccharide depending on the substrate (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang, Guofeng, et al. "Hyaluronan and hyaluronan synthases expression and localization in embryonic mouse molars." Journal of Molecular Histology: 1-8.Read more>>
  • Pascual, Gloria. et al. Dietary palmitic acid promotes a prometastatic memory via Schwann cells. Nature. 2021 Nov;:1-6Read more>>
  • Romana ?nov. et al. The hyaluronan metabolism in the UV-irradiated human epidermis and the relevance of in vitro epidermal models. EXP DERMATOL. 2023 JulRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat spleen labeled with Anti-HAS1 Polyclonal Antibody, Unconjugated (bs-2946R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


U-87MG (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HAS1 Antibody (bs-2946R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).