bs-2925R
[Primary Antibody]
Klotho Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Klotho
Immunogen Range: 401-500/1012
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9365
Swiss Prot: Q9UEF7
Source: KLH conjugated synthetic peptide derived from human Klotho
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
Size: 100ul
Concentration: 1ug/ul
Applications:
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
Predicted Molecular Weight: 113
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Prud'homme, Gérald J., et al. "The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells." Biochemical and Biophysical Research Communications (2017)Read more>>
- Ni et al. Adipose-Derived Mesenchymal Stem Cells Transplantation Alleviates Renal Injury in Streptozotocin-Induced Diabetic Nephropathy. (2015) J.Histochem.Cytochem. 63:842-53Read more>>
- Stenhouse Claire. et al. Novel Mineral Regulatory Pathways in Ovine Pregnancy: I. Phosphate, Klotho Signaling, and Sodium Dependent Phosphate Transporters. Biol Reprod. 2021 FebRead more>>
- Xiao-Tan Zhang. et al. Baicalin reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal injury in type 1 diabetic mouse model. Cell Cycle. 2020;19(23):3329-3347Read more>>
- Nianbin Song. et al. Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cell Immunol. 2020 Nov;357:10421Read more>>
- Amelia K. Fotheringham. et al. Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structure. Iscience. 2021 Oct;:103308Read more>>
- Stenhouse Claire. et al. Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling. Biol Reprod. 2022 FebRead more>>
- Li Lei. et al. LncRNA 152 attenuates lipopolysaccharide-induced acute kidney injury in rats by regulating the FGF23/Klotho/MAPK axis. NEPHROLOGY. 2023 SepRead more>>
- Xiangjuan Guo. et al. Network Pharmacology and Transcriptomics to Explore the Pharmacological Mechanisms of 20(S)-Protopanaxatriol in the Treatment of Depression. INT J MOL SCI. 2024 Jul; 25(14): 7574Read more>>
- Xi-Kun Yuan. et al. Effects of Life-Long Exercise on Age-Related Inflammation, Apoptosis, Oxidative Stress, Ferroptosis Markers, and NRF2/KAEP 1/Klotho Pathway in Rat Kidneys. PHYSIOL RES. 2024 Aug 31;73(4):577-591Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Klotho Polyclonal Antibody, Unconjugated (bs-2925R) at 1:400 overnight at 4°C, DAB staining.