bs-2912R-BF405
[Conjugated Primary Antibody]
MAP1A/MAP1B LC3 A/B Antibody, AbBy Fluor® 405 Conjugated
DATASHEET
Host: Rabbit
Target Protein: MAP1A/MAP1B LC3 A/B
Immunogen Range: 21-121/121
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 81631, 84557
Source: KLH conjugated synthetic peptide derived from human MAP1A/MAP1B LC3 B
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy.
Conjugation: AbBy Fluor® 405
Excitation/ Emission: 401nm/421nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 13
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Lin, Chia-Fa, et al. "Quercetin-Rich Guava (Psidium guajava) Juice in Combination with Trehalose Reduces Autophagy, Apoptosis and Pyroptosis Formation in the Kidney and Pancreas of Type II Diabetic Rats." Molecules21.3 (2016): 334.Read more>>
- Liu B et al. Effects of Autophagy on Synaptic-Plasticity-Related Protein Expression in the Hippocampus CA1 of a Rat Model of Vascular Dementia. Neurosci Lett. 2019 Jun 1;707:134312.Read more>>
- Wang WJ et al. Endometrial TGF-β, IL-10, IL-17 and autophagy are dysregulated in women with recurrent implantation failure with chronic endometritis. Reprod Biol Endocrinol. 2019 Jan 3;17(1):2.Read more>>
- Yin X et al. Lithium facilitates removal of misfolded proteins and attenuated faulty interaction between mutant SOD1 and p-CREB (Ser133) through enhanced autophagy in mutant hSOD1G93A transfected neuronal cell lines. Mol Biol Rep. 2019 Sep 16. Read more>>
- Zhang P et al. Human metapneumovirus induces autophagy through JNK and MEK/ERK pathway. assets.researchsquare.2019.Read more>>
- Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKII-associated activation of autophagy. Cell Biosci. 2020 Jul 30;10:90.Read more>>
- Liwen Luo. et al. Cartilage endplate stem cells inhibit intervertebral disc degeneration by releasing exosomes to nucleus pulposus cells to activate Akt/autophagy. Stem Cells. 2021 Jan 18Read more>>
- Jihang Dai. et al. Glabridin inhibits osteoarthritis development by protecting chondrocytes against oxidative stress, apoptosis and promoting mTOR mediated autophagy. Life Sci. 2021 Mar;268:118992Read more>>
- Zihua Liu. et al. Overexpression of thioredoxin reductase 1 can reduce DNA damage, mitochondrial autophagy and endoplasmic reticulum stress in Parkinson's disease. 2020 Nov 23Read more>>
- Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306Read more>>
- Ruiqiao Guan. et al. Electroacupuncture at GV20?GB7 regulates mitophagy to protect against neurological deficits following intracerebral hemorrhage via inhibition of apoptosis. Mol Med Rep. 2021 Jul;24(1):1-15Read more>>
- Jinjin Shi. et al. Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. 2021 Oct 2Read more>>
- Jinbo Li. et al. Autophagy inhibition recovers deficient ICD-based cancer immunotherapy. BIOMATERIALS. 2022 Aug;287:121651Read more>>
- Zhao-Jie Ji. et al. Neuroprotective Effect of Taohong Siwu Decoction on Cerebral Ischemia/Reperfusion Injury via Mitophagy-NLRP3 Inflammasome Pathway. FRONT PHARMACOL. 2022; 13: 910217Read more>>
- Zhang, Kai. et al. Long-Term Copper Exposure Induced Excessive Autophagy of the Porcine Spleen. BIOL TRACE ELEM RES. 2022 Jul;:1-9Read more>>
- Yu-Mei Jia. et al. Effects on Autophagy of Moxibustion at Governor Vessel Acupoints in APP/PS1double-Transgenic Alzheimers Disease Mice through the lncRNA Six3os1/miR-511-3p/AKT3 Molecular Axis. EVID-BASED COMPL ALT. 2022 Oct 05;2022:3881962Read more>>
- Tengyun Yang. et al. MicroRNA-146a-5p alleviates the pathogenesis of osteoarthritis by inhibiting SDF-1/CXCR4-induced chondrocyte autophagy. INT IMMUNOPHARMACOL. 2023 Apr;117:109938Read more>>
- Lulu Li. et al. Linarin Protects Against CCl4-Induced Acute Liver Injury via Activating Autophagy and Inhibiting the Inflammatory Response: Involving the TLR4/MAPK/Nrf2 Pathway. DRUG DES DEV THER. 2023 Dec 01Read more>>
- Xingbo Wang. et al. Construction of a cascade nanosystem to implement indirect and direct cell modulation for tumor microenvironment immunostimulation. CHEM ENG J. 2024 Apr;485:150141Read more>>
- Xiaoxiao Shan. et al. Kai-Xin-San ameliorates Alzheimer's disease-related neuropathology and cognitive impairment in APP/PS1 mice via the mitochondrial autophagy-NLRP3 inflammasome pathway. J ETHNOPHARMACOL. 2024 Apr;:118145Read more>>
- Yang Xu. et al. Dietary salt promotes cognition impairment through GLP-1R/mTOR/p70S6K signaling pathway. SCI REP-UK. 2024 Apr;14(1):1-16Read more>>
- Ying Liu. et al. Acacetin inhibits activation of microglia to improve neuroinflammation after subarachnoid hemorrhage through the PERK signaling pathway mediated autophagy. HUM EXP TOXICOL. ;()Read more>>
- Xiu-Li Zhang. et al. MCT1-mediated transport of valeric acid promotes porcine preimplantation embryo development by improving mitochondrial function and inhibiting the autophagic AMPK-ULK1 pathway. THERIOGENOLOGY. 2024 Sep;225:152Read more>>
- Jinfeng Shang. et al. Naoxintong capsule accelerates mitophagy in cerebral ischemia-reperfusion injury via TP53/PINK1/PRKN pathway based on network pharmacology analysis and experimental validation. J ETHNOPHARMACOL. 2024 Aug;:118721Read more>>
VALIDATION IMAGES