Host: Rabbit
Target Protein: Lyn
Specificity: This protein is known to be similar in amino acid sequence to HCK. Therefore, cross-reactivity with this homologous protein may be observed
Immunogen Range: 201-300/512
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4067
Swiss Prot: P07948
Source: KLH conjugated synthetic peptide derived from human Lyn
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 58
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Iriyama et al. Lyn, a tyrosine kinase closely linked to the differentiation status of primary acute myeloid leukemia blasts, associates with negative regulation of all-trans retinoic acid (ATRA) and dihydroxyvitamin D3 (VD3)-induced HL-60 cells differentiation. (2016) Cancer.Cell.Int. 16:37Read more>>
- Fu S et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling.Int Immunopharmacol. 2019 Jun;71:1-6. Read more>>
- Xiangyu Liang. et al. Effects and potential mechanisms of Saposhnikovia divaricata (Turcz.) Schischk. On type I allergy and pseudoallergic reactions in vitro and in vivo. J ETHNOPHARMACOL. 2024 Jan;318:116942Read more>>
Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti Lyn Polyclonal Antibody, PE conjugated (bs-2906R-PE) at 1:200 dilution for 60 minutes at 37°C.
Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Anti Lyn Polyclonal Antibody, PE conjugated (bs-2906R-PE) at 1:200 dilution for 60 minutes at 37°C.
A431 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lyn Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated (bs-2906R-AF64) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Lane 1: Mouse SP2/0 cell lysates probed with Lyn Polyclonal Antibody, Unconjugated (bs-2906R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Lymph node tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human Raji cell lysates probed with Lyn Polyclonal Antibody, Unconjugated (bs-2906R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.