bs-2887R [Primary Antibody]
CYP2C9 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CYP2C9

Immunogen Range: 396-410/490


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1559

Swiss Prot: P11712

Source: KLH conjugated synthetic peptide derived from human CYP2C9

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang et al. Hyperhomocysteinemia results from and promotes hepatocellular carcinoma via CYP450 metabolism by CYP2J2 DNA methylation. (2017) Oncotarge. 8:15377-15392Read more>>
  • Kai Wang. et al. Drug-drug interactions induced by Linderane based on mechanism-based inactivation of CYP2C9 and the molecular mechanisms. Bioorg Chem. 2022 Jan;118:105478Read more>>
  • Wang Shanshan. et al. Gut microbiota and host cytochrome P450 characteristics in the pseudo germ-free model: co-contributors to a diverse metabolic landscape. GUT PATHOG. 2023 Dec;15(1):1-14Read more>>
  • Huan Yang. et al. Heat stress induces ferroptosis of porcine Sertoli cells by enhancing CYP2C9-Ras- JNK axis. THERIOGENOLOGY. 2024 Feb;215:281Read more>>
  • Wang Yuying. et al. Chemotherapy-induced acetylation of ACLY by NAT10 promotes its nuclear accumulation and acetyl-CoA production to drive chemoresistance in hepatocellular carcinoma. CELL DEATH DIS. 2024 Jul;15(7):1-17Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-CYP2C9 Polyclonal Antibody, Unconjugated (bs-2887R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Stomach tissue lysates probed with CYP2C9 Polyclonal Antibody, Unconjugated (bs-2887R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.