bs-2882R-FITC [Conjugated Primary Antibody]
LPA3 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
[email protected]
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+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LPA3

Immunogen Range: 101-200/353


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23566

Swiss Prot: Q9UBY5

Source: KLH conjugated synthetic peptide derived from human EDG7/LPA3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. May play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Linmu, et al. "Lysophosphatidic acid individually induces macrophage-derived foam cell formation by blocking the expression of SRBI." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Chen L et al. Lysophosphatidic acid decreased macrophage foam cell migration correlated with downregulation of fucosyltransferase 8 via HNF1α. Atherosclerosis. 2019 Sep 11;290:19-30. Read more>>
  • Shannon Weber Doutt. et al. LPAR1 and aberrantly expressed LPAR3 differentially promote the migration and proliferation of malignant peripheral nerve sheath tumor cells. GLIA. 2022 NovRead more>>
VALIDATION IMAGES

293T cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with LPA3 Polyclonal Antibody(bs-2882R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).