bs-2843R [Primary Antibody]
phospho-Tau (Thr217) polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: phospho-Tau (Thr217)

Modification Site: Thr217

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4137

Swiss Prot: P10636

Source: KLH conjugated Synthesised phosphopeptide derived from human Tau around the phosphorylation site of Thr4217)

Purification: affinity Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 52/79


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Jurkat cell lysates probed with phospho-Tau (Thr217) Polyclonal Antibody, Unconjugated (bs-2843R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Human glioblastoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with phospho-Tau (Thr217) Polyclonal Antibody, Unconjugated (bs-2843R) at 1:200 overnight at 4°C, DAB staining.