bs-28027R [Primary Antibody]
P38 MAPK/ MAPK14 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P38 MAPK/ MAPK14

Immunogen Range: 121-220/360


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1432

Swiss Prot: Q16539

Source: KLH conjugated synthetic peptide derived from human P38 MAPK/ MAPK14

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF()

Predicted Molecular Weight: 41 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chi Q et al. Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK/NF-κB Pathway in Broiler Spleen. Oxid Med Cell Longev. 2019 Jul 31;2019:8061823. Read more>>
  • Li QH et al. Effect of heat stress on mitogen-activated protein kinases in the hypothalamic− pituitary− gonadal axis of developing Wenchang chicks. Poultry Science.2019.Read more>>
  • Pan W et al. MicroRNA-124 alleviates the lung injury in mice with septic shock through inhibiting the activation of the MAPK signaling pathway by downregulating MAPK14. Int Immunopharmacol. 2019 Aug 30;76:105835. Read more>>
  • Liu F et al. Anticancer targets and mechanisms of calycosin to treat nasopharyngeal carcinoma. Biofactors. 2020 Jul;46(4):675-684.Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127Read more>>
  • Manman Shen. et al. Effects of quercetin on granulosa cells from pre-hierarchical follicles by modulating MAPK signaling pathway in chicken. POULTRY SCIENCE. 2023 Apr;:102736Read more>>
  • Wen Yu Zhang. et al. AMPK regulates immature boar Sertoli cell proliferation through affecting CDK4/Cyclin D3 pathway and mitochondrial function. THERIOGENOLOGY. 2024 Aug;224:9Read more>>
  • Chenhao Xu. et al. Human umbilical cord mesenchymal stem cells alleviate fatty liver ischemia-reperfusion injury by activating autophagy through upregulation of IFN. CELL BIOCHEM FUNCT. 2024 Jun;42(4):e404Read more>>
  • Yuquan Guo. et al. Revealing the Mechanism: The Influence of Baicalin on M1/M2 and Th1/Th2 Imbalances in Mycoplasma gallisepticum Infection. POULTRY SCI. 2024 Jul;:104145Read more>>
VALIDATION IMAGES

Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Rat Muscle tissue lysates probed with P38 MAPK/ MAPK14 Polyclonal Antibody, Unconjugated (bs-28027R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.