Host: Rabbit
Target Protein: KLF2
Immunogen Range: 251-355/355
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10365
Swiss Prot: Q9Y5W3
Source: KLH conjugated synthetic peptide derived from human KLF2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 37
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Wang, Y. L., et al. "Effects of Artesunate and Ursolic Acid on Hyperlipidemia and its Complications in Rabbit." European Journal of Pharmaceutical Sciences (2013).Read more>>
- Zhang P, Ha T, Larouche M, Swanson D, Goldowitz D (2015) Kruppel-Like Factor 4 Regulates Granule Cell Pax6 Expression and Cell Proliferation in Early Cerebellar Development. PLoS ONE 10(7): e0134390.Read more>>
- Hong Jin. et al. BDNF corrects NLRP3 inflammasome-induced pyroptosis and glucose metabolism reprogramming through KLF2/HK1 pathway in vascular endothelial cells. Cell Signal. 2021 Feb;78:109843Read more>>
- Xiaoyan Hu. et al. Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Mol Med Rep. 2020 Sep;22(3):2003-2011Read more>>
- Cao, Yaochen. et al. Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis. CELL BIOL TOXICOL. 2022 May;:1-17Read more>>
- Wang, Yiying. et al. mTOR contributes to endothelium-dependent vasorelaxation by promoting eNOS expression and preventing eNOS uncoupling. COMMUN BIOL. 2022 Jul;5(1):1-14Read more>>
- Yanda Zhang. et al. Free fatty acids induce coronary microvascular dysfunction via inhibition of the AMPK/KLF2/eNOS signaling pathway. INT J MOL MED. 2023 Apr;51(4):1-14Read more>>
- Yuying Kou. et al. Simvastatin inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma through KLF2 signal. J ORAL BIOSCI. 2023 AugRead more>>
Formalin-fixed and paraffin-embedded Rat Lung tissue labeled with KLF2 Polyclonal Antibody, Cy5 Conjugated (bs-2772R-Cy5) at 1:200 for 40 minutes at 37°C.
Mouse embryo lysates probed with Anti KLF2 Polyclonal Antibody, Unconjugated (bs-2772R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
Formalin-fixed and paraffin-embedded mouse embryo labeled with Anti-KLF2 Polyclonal Antibody, Unconjugated(bs-2772R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
MCF-7 cells were stained with KLF2 Polyclonal Antibody, Unconjugated (bs-2772R) at 1:200 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with KLF2 Polyclonal Antibody, Unconjugated (bs-2772R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with KLF2 Polyclonal Antibody, Unconjugated (bs-2772R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (bs-2772R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.