DATASHEET
Host:
Rabbit
Target Protein:
ACACA
Immunogen Range:
2307-2355/2458
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
31
Swiss Prot:
Q13085
Source:
KLH conjugated synthetic peptide derived from human ACACA
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
PRODUCT SPECIFIC PUBLICATIONS
- Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29. Read more>>
- Wang J et al. Novel anti-obesity peptide (RLLPH) derived from hazelnut (Corylus heterophylla Fisch) protein hydrolysates inhibits adipogenesis in 3T3-L1 adipocytes by regulating adipogenic transcription factors and adenosine monophosphate-activated protein kinase (AMPK) activation. J Biosci Bioeng. 2019 Oct 17. pii: S1389-1723(19)30741-8. Read more>>
- Sun W et al. Baicalein reduced hepatic fat accumulation by activating AMPK in oleic acid-induced HepG2 cells and high-fat diet-induced non-insulin resistant mice. Food Funct. 2020 Jan 7.Read more>>
- Pan Y et al. Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Int J Biol Macromol. 2019 Nov 8. pii: S0141-8130(19)37563-4. Read more>>
- Wen-Long Sun. et al. Myricetin supplementation decreases hepatic lipid synthesis and inflammation by modulating gut microbiota. Cell Rep. 2021 Aug;36:109641Read more>>
- Kaiyang Chen. et al. Oligosaccharide and short-chain fatty acid: A double-edged sword in obese mice by regulating food intake and fat synthesis. FOOD RES INT. 2022 Sep;159:111619Read more>>
VALIDATION IMAGES
NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ACACA) polyclonal Antibody, Unconjugated (bs-2745R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.