Host: Rabbit
Target Protein: IRS-1 Ser307
Specificity: This phosphorylation site is homologous to Ser302 in Mouse and Rat.
Modification Site: Ser307
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3667
Swiss Prot: P35568
Source: KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser307 [TE(p-S)IT]
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 132
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Luo, Cheng, et al. "Kaempferol alleviates insulin resistance via hepatic IKK/NF-κB signal in type 2 diabetic rats." International Immunopharmacology 28.1 (2015): 744-750.Read more>>
- Gao, Sujie, et al. "Propofol inhibits growth of neurons through regulating insulin receptor and insulin-like growth factor-1 receptor." Int J Clin Exp Pathol 9.7 (2016): 6785-6794.Read more>>
- Zhang et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. (2018) Molecules. 23 Read more>>
- Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.Read more>>
- Yang Hui. et al. Activation of Hippocampal IR/IRS-1 Signaling Contributes to the Treatment with Zuogui Jiangtang Jieyu Decoction on the Diabetes-Related Depression. Evid-Based Compl Alt. 2021;2021:6688723Read more>>
- Wang, Hongyan. et al. Protection of pancreatic -cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3 and STAT3/Cyp-D signaling pathways. 2021 Aug 28Read more>>
Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-phospho-IRS-1(Ser307) Polyclonal Antibody, Unconjugated (bs-2736R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRS-1(Ser307) Polyclonal Antibody(bs-2736R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).