VALIDATION IMAGES
Formalin-fixed and paraffin embedded human kidney labeled with Anti-GST Polyclonal Antibody, Unconjugated (bs-2735R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-GST Polyclonal Antibody, Unconjugated (bs-2735R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Paraformaldehyde-fixed, paraffin embedded (Rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (Mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal rabbit serum) at 37°C for 30min; Incubation: Anti-GST Antibody, conjugated (bs-2735R-AF594) at 1:200 overnight at 4°C; DAPI (blue, C02-04002) was used to stain the cell nuclei.