bs-2720R-BF488 [Conjugated Primary Antibody]
Akt(Thr308) Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Akt Thr308

Modification Site: Thr308

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 207

Source: KLH conjugated synthetic phosphopeptide derived from human Akt around the phosphorylation site of Thr308

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li et al. The dual PI3K/mTOR inhibitor NVP-BEZ235 inhibits proliferation and induces apoptosis of burkitt lymphoma cells. (2015) Cancer.Cell.Int. 15:65Read more>>
  • Ou X et al. Desumoylating Isopeptidase 2 (DESI2) Inhibits Proliferation and Promotes Apoptosis of Pancreatic Cancer Cells through Regulating PI3K/AKT/mTOR Signaling Pathway. (2018)Pathol. Oncol. Res. Nov 8.Read more>>
  • Zheng R et al. Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears. Cell Tissue Res. 2019 Apr 24.Read more>>
  • Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22. Read more>>
  • Wang Y et al. Targeting the miR-122/PKM2 autophagy axis relieves arsenic stress。 Journal of Hazardous Materials. 2019 Sep.Read more>>
  • X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106.Read more>>
  • Li X et al. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Biosci Rep. 2020 Jun 26;40(6):BSR20191327.Read more>>
  • Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
  • Jisheng Wang. et al. To study the mechanism of Cuscuta chinensis Lam. And Lycium barbarum L. in the treatment of asthenospermia based on network pharmacology. J Ethnopharmacol. 2021 Apr;270:11379Read more>>
  • Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51C6Read more>>
  • Yu Wang. et al. Zinc application alleviates the adverse renal effects of arsenic stress in a protein quality control way in common carp. Environ Res. 2020 Dec;191:110063Read more>>
  • Dezhi Wu. et al. Long non?coding RNA maternally expressed gene?3 affects cell proliferation, apoptosis and migration by targeting the microRNA?9?5p/midkine axis and activating the phosphoinositide?dependent kinase/AKT pathway in hepatocellular carcinoma. Oncol Lett. 2021 May;21(5):1-8Read more>>
  • Wang Ji-sheng. et al. A Bioinformatic Investigation of the Mechanism Underlying Migraine-Induced Erectile Dysfunction. Biomed Res Int. 2021;2021:6674643Read more>>
  • Yu Zheng. et al. Advanced oxidation protein products trigger apoptosis and block epithelial?to?mesenchymal transition in crypt epithelial cells. Exp Ther Med. 2021 Aug;22(2):1-6Read more>>
  • Ning Zhong. et al. Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31. 2021 Sep 3Read more>>
  • Shi, Linjie. et al. Memory regulation in feeding habit transformation to dead prey fish of Chinese perch (Siniperca chuatsi). 2021 Sep 28Read more>>
  • Qi Hao. et al. Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway. Brit J Nutr. 2021 Dec;:1-27Read more>>
  • Ji-sheng Wang. et al. Explore the effects of pulmonary fibrosis on sperm quality and the role of the PI3K/Akt pathway based on rat model. 2021 Dec 21Read more>>
  • Yongxin Guo. et al. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. INT J MOL SCI. 2022 Jan;23(21):13152Read more>>
  • Rui Sun. et al. Construction of crizotinib resistant models with CD74-ROS1 D2033N and CD74-ROS1 S1986F point mutations to explore resistance mechanism and treatment strategy. CELL SIGNAL. 2023 Jan;101:110497Read more>>
  • Hui Tong. et al. Silencing of KNTC1 inhibits hepatocellular carcinoma cells progression via suppressing PI3K/Akt pathway. CELL SIGNAL. 2022 Oct;:110498Read more>>
  • Mengqi Yu. et al. miR-27a Targeting PIK3R3 Regulates the Proliferation and Apoptosis of Sheep Hair Follicle Stem Cells. ANIMALS. 2023 Jan;13(1):141Read more>>
  • Fan Zhiqin. et al. Phosphorylation of AKT by lysyl oxidase-like 2 activates the PI3K/AKT signaling pathway to promote proliferation, invasion and metastasis in esophageal squamous carcinoma. CLIN TRANSL ONCOL. 2023 Mar;:1-12Read more>>
  • Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26Read more>>
  • Qi Zhao. et al. Investigation of the Underlying Mechanism of Depression on Male Infertility: A Bioinformatics and Experimental Research Study. ANDROLOGIA. 2023;2023:2820367Read more>>
  • Yuan Li. et al. A Biomimetic Peptide Functions as Specific Extracellular Matrix for Quiescence of Stem Cells against Intervertebral Disc Degeneration. SMALL. 2023 Jul;:2300578Read more>>
  • Yao Li. et al. Syringic acid (SA) inhibits the IL-1-induced inflammation in mice chondrocytes and ameliorates the progression of osteoarthritis via the PTEN/AKT/NF-B pathway. J FUNCT FOODS. 2023 Aug;107:105683Read more>>
  • Yana Lv. et al. Unveiling the potential mechanisms of Amomi fructus against gastric ulcers via integrating network pharmacology and in vivo experiments. J ETHNOPHARMACOL. 2023 Sep;:117179Read more>>
  • Hao-Xuan Du. et al. Eukaryotic translation initiation factor 2 kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization. ONCOL LETT. 2023 Nov;26(5):1-21Read more>>
  • Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
  • Li-Mei Wang. et al. In Vivo and In Vitro Study on the Mechanism of Anticervical Cancer Effects of Corilagin in Mice. J FOOD BIOCHEM. 2024;2024:48229Read more>>
  • Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-B-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11Read more>>