Host: Rabbit
Target Protein: TLR9
Immunogen Range: 951-1032/1032
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54106
Swiss Prot: Q9NR96
Source: KLH conjugated synthetic peptide derived from human TLR9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 113
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Fu, Juanli, et al. "Tetrachlorobenzoquinone exerts neurological pro-inflammatory activity by promoting HMGB1 release, which induces TLR4 clustering within the lipid raft." Toxicological Sciences (2016): kfw124.Read more>>
- Dong W et al. Polychlorinated biphenyl quinone induces caspase 1-mediated pyroptosis through the induction of pro-inflammatory HMGB1-TLR4-NLRP3-GSDMD signal axis. Chem Res Toxicol. 2019 Apr 23. Read more>>
- Xuting Liu. et al. Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1/TLR4/MYD88/NF-kb signaling pathway in HUVEC cells. J Hazard Mater. 2021 Feb;404:12405Read more>>
- Dai Miyazaki. et al. Role Played by Receptors for Advanced Glycosylation End Products in Corneal Endothelial Cells after HSV-1 Infection. Int J Mol Sci. 2021 Jan;22(11):5833Read more>>
- Ying Xia. et al. SB431542 alleviates lupus nephritis by regulating B cells and inhibiting the TLR9/TGF1/PDGFB signaling. J AUTOIMMUN. 2022 Oct;132:102894Read more>>
Mouse splenocytes probed with TLR9 Polyclonal Antibody, Unconjugated (bs-2717R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti TLR9/CD289 Polyclonal Antibody, Unconjugated (bs-2717R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TLR9) polyclonal Antibody, Unconjugated (bs-2717R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.