bs-2706R [Primary Antibody]
SIGLEC10 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SIGLEC10

Immunogen Range: 321-420/697


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 89790

Swiss Prot: Q96LC7

Source: KLH conjugated synthetic peptide derived from human SIGLEC10

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

SIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation. The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 77


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiao Han. et al. Pyrotinib Targeted EGFR-STAT3/CD24 Loop-Mediated Cell Viability in TSC. CELLS-BASEL. 2022 Jan;11(19):3064Read more>>
VALIDATION IMAGES

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human Jurkat cell lysates probed with SIGLEC10 Polyclonal Antibody, Unconjugated (bs-2706R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.