DATASHEET
Host:
Rabbit
Target Protein:
Semaphorin 7A
Immunogen Range:
351-450/666
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
8482
Swiss Prot:
O75326
Source:
KLH conjugated synthetic peptide derived from human Semaphorin 7A
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SEMA7A/CD108 Polyclonal Antibody, Unconjugated(bs-2702R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: mouse brain lysates Lane 2: mouse lung lysates probed with Anti Sema7A/CD108 Polyclonal Antibody, Unconjugated (bs-2702R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 66kD. Observed band size: 66kD.
25 ug total protein per lane of various lysates (see on figure) probed with CD108 polyclonal antibody, unconjugated (bs-2702R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.