bs-2684R [Primary Antibody]
GPNMB Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPNMB

Immunogen Range: 81-180/572


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10457

Swiss Prot: Q14956

Source: KLH conjugated synthetic peptide derived from human GPNMB

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function." Journal of Biological Chemistry (2015): jbc-M114.Read more>>
  • Arosarena, Oneida A., et al. "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." Journal of Cellular Physiology (2016).Read more>>
  • Frara, Nagat, et al. "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." BMC Musculoskeletal Disorders 17.1 (2016): 1.Read more>>
  • Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015.Read more>>
  • Oyewumi et al. Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells. (2016) Oncotarge. 7:13932-44Read more>>
  • Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73Read more>>
  • Suda, Masayoshi. et al. Glycoprotein nonmetastatic melanoma protein B regulates lysosomal integrity and lifespan of senescent cells. SCI REP-UK. 2022 Apr;12(1):1-14Read more>>
  • Carmen H?rdtner. et al. A comparative gene expression matrix in Apoe-deficient mice identifies unique and atherosclerotic disease stage-specific gene regulation patterns in monocytes and macrophages. ATHEROSCLEROSIS. 2023 Apr;371:1Read more>>
  • Xuejing Hou. et al. Glycoprotein Non-metastatic Melanoma Protein B (GPNMB) Protects Against Neuroinflammation and Neuronal Loss in Pilocarpine-induced Epilepsy via the Regulation of Microglial Polarization. NEUROSCIENCE. 2024 MayRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded rat lung labeled with Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated (bs-2684R) at 1:200 followed by the conjugated secondary antibody and DAB staining.


PANC-1 cell lysates probed with GPNMB Polyclonal Antibody, Unconjugated (bs-2684R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Recombinant human GPNMB protein, C-His (HEK293)(bs-43555P) probed with GPNMB Polyclonal Antibody, Unconjugated (bs-2684R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.