Host: Rabbit
Target Protein: ELAVL1
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1994
Swiss Prot: Q15717
Source: KLH conjugated synthetic peptide derived from human ELAVL1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
ELISA(ELISA=1:5000-10000)
FCM(FCM=1:50)
Predicted Molecular Weight: 36
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ELAVL1 Antibody(bs-2651R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).