bs-2625R [Primary Antibody]
IL-22BP/IL22RA2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL22RA2

Immunogen Range: 165-263/263


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 116379

Swiss Prot: Q969J5

Source: KLH conjugated synthetic peptide derived from human IL22RA2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

IL22RA2 is a member of the class II cytokine receptor family. The soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. IL22RA2 may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Isoform 2 is a receptor for IL22, and may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Isoform 1 may play a role in establishing and maintaining a successful pregnancy.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 28(hu)/25(mo, ra


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Almishri, Wagdi. Regulatory Immune-Responses Occurring within the Liver Downstream of Hepatic iNKTcell activation. Diss. University of Calgary, 2015.Read more>>
  • Wang, Xiaobai. et al. Relationship between IL-22 and IL-22BP in diabetic cognitive dysfunction. ACTA DIABETOL. 2023 Jan;:1-14Read more>>
VALIDATION IMAGES

Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n


Mouse splenocytes probed with IL-22BP/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-22BP/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Lane 1: Rat testis lysates; probed with IL-22BP/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Mouse Spleen Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with IL-22BP/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).